Part:BBa_K245108:Design
KSI-DP-P2/33-LL37-ELST-GCN-ELST
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 375
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 55
Illegal NgoMIV site found at 313
Illegal NgoMIV site found at 412 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. KSI-DP-P2/33 basic part was added to other parts by cutting front inserts with EcoRI and SpeI and front vectors with EcoRI and XbaI, thus forming scar Xba/Spe NgoMIV (BBa_K245080) beween KSI-DP-P2/33 and another part. This was necessary since BB-NIC-III-HisN vector containing KSI-DP-P2/33 (BBa_K245113) does not contain NgoMIV and BspEI restriction sites in its multiple cloning site (which were usually used to assemble composite parts). There is additional NgoMIV restriction site in the scar since BB-NIC-III-HisN vectors containing other parts (iGEM 2009, team Slovenia) have NgoMIV and BspEI restriction sites in their multiple cloning site (XbaI-NgoMIV-part-BspEI-SpeI).
Source
Composed from KSI-DP-P2/33 and LL37-ELST-GCN-ELST intermediate parts according to assembly standard 37.